BAX System Real-Time PCR Assay for E. coli O157-H7
Hygiena | BAX® System Real-Time PCR Assay for E. coli O157-H7
E. coli O157:H7 is a recognized foodborne pathogen found in raw beef and more recently in produce. It can cause serious and sometimes fatal illness at a very low infectious dose (as few as 10 organisms). The organism is often difficult to detect with traditional culture methods, especially where low levels of the target must be isolated from high levels of competing background flora. The BAX® System uses DNA-based technology to provide same-day results that are reliable and reproducible, allowing food producers and processors to quickly and accurately release safe products to the market.
Benefits
- Speedy results help you make product decisions the same day
- Simplified DNA extraction - no separate cell concentration steps
- Easy operation of automated instrument does not require advanced skills
- PCR tablets conveniently packaged in individual PCR tubes
- Highly accurate - detects all known E. coli 0157:H7. including rough strains
- Reliable - developed in collaboration with USDA ARS
- Same-day results as little as 10 hours for some matrices
Specifications
- Real-time
- Target: E. coli O157:H7
- Matrices: Raw ground beef, beef trim, produce
- Enrichment time: 8-10 hours, depending on food type, sample volume and enrichment media
- PCR time: 55 minutes
- Detection level: 10^4 cfu/mL, after enrichment
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Validations:
- AOAC Research Institute - Performance Tested Method #031002 - validated on raw ground beef, beef trim, spinach and lettuce
- AFNOR - QUA-18/07-07/10 - validated on raw beef and vegetables
- Health Canada - MFLP-76 - validated on raw ground beef and beef trim
Catalog No.
| Legacy Order Code | Hygiena Product Code | Description | Quantity |
| D14203648 | KIT2000 | BAX® System Real-time Assay for E. coli O157:H7 | 96 tests per kit |
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Resources
Instructions
Safety Data Sheets (SDS)
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